FIGURE 5.

ARTN enhances the CSC-like population in ER+MC cells. A, MCF-7 cells with forced expression of ARTN were seeded in ultra low attachment plates in mammospheric media. After 10 days, growth was measured by alamarBlue. B, ARTN increases the ALDH1+ cell population in MCF-7 cells. Shown is a representative bar graph analysis using the FACS analysis of the Aldefluor assay with MCF-7-VEC and MCF-7-ARTN cells. Cells were incubated with Aldefluor substrate (BAAA, BODIPY®-aminoacetaldehyde) to define the Aldefluor-positive, and a specific inhibitor of ALDH1, diethylaminobenzaldehyde (DEAB), was used to establish the base-line fluorescence. Flow cytometry plots indicate side scatter (SSC) versus fluorescence intensity. **, p < 0.01.