Skip to main content
. 2012 Oct 24;287(51):42502–42515. doi: 10.1074/jbc.M112.365163

FIGURE 6.

FIGURE 6.

ARTN increases BCL-2 expression to promote a CSC like phenotype in ER-MC cells. A, Western blot determination of BCL-2 protein levels in MDA-MB-231 and BT549 cells with forced expression and depletion of ARTN is shown. β-Actin was used as a loading control for cell lysates. The sizes of the detected protein bands in kDa are shown on the right. B, Western blot determination of BCL-2 protein levels in MDA-MB-231 cells with forced expression of ARTN± siRNA to TWIST1 is shown. β-Actin was used as loading control for cell lysates. The sizes of the detected protein bands in kDa are shown on the right. C, Western blot determination of TWIST1 and BCL-2 protein levels in MDA-MB-231 and BT549 cells with forced expression of ARTN grown under mammospheric conditions is shown. β-Actin was used as loading control for cell lysates. The sizes of the detected protein bands in kDa are shown on the right. D, mammospheric growth of MDA-MB-231 and BT549 cells with forced expression of ARTN was determined ± YC137 (5 μm). DMSO-treated VEC cells are presented as 100%. E, mammospheric growth of MDA-MB-231 and BT549 cells with forced expression of ARTN was determined ± siRNA to TWIST1. siCONT-treated VEC cells are presented as 100%. F, the Aldefluor assay was performed in MDA-MB-231 cells with forced expression of ARTN ± siRNA to TWIST1. Flow cytometry plots indicate side scatter (SSC) versus fluorescence intensity. **, p < 0.01.