FIGURE 2.

Peroxidase activities of the wild type and mutant Prx2s. To determine peroxidase activities, the residual H₂O₂ in the reaction mixtures were measured at the indicated times using ferrous oxidation xylenol 1 reagent. A, none (●), Prx2 (○), TrxA/TrR (□), or Prx2 and TrxA/TrR (▾) were added to the reaction mixture containing NADPH and H₂O₂. Residual tBHP in the reaction mixture containing Prx2, TrxA/TrR, NADPH, and tBHP in place of H₂O₂, were presented (▵). B, none (●), wild-type Prx2 and TrxA/TrR (▾), Prx2-C50S and TrxA/TrR (■), or Prx2-C171S and TrxA/TrR (□) were added to the reaction mixture containing NADPH and H₂O₂. The S.E. were too small to denote by error bars.