FIGURE 5.

Expression of prx1 and prx2 under oxidative stress. RNAs and proteins were extracted from the cultures grown anaerobically (A and B) and aerobically (C–E), after being exposed to various levels of H₂O₂ for 1 min (A and B) and 5 min (C-E) as indicated. For A, B, and E, RNAs were resolved and hybridized to a ³²P-labeled DNA probe corresponding to the internal coding regions of prx1 (PRX1P, A and E) or prx2 (PRX2P, B). The RNA size markers (Invitrogen) and prx1 and prx2 transcripts are shown in kilobases. For C and D, total proteins were resolved on reducing SDS-PAGE, and immunoblotted using the rabbit anti-Prx1 (C) and Prx2 (D) antibody. prx2, prx2 mutant; prx1, prx1 mutant. Western blots are presented as described in the legend to Fig. 4.