TABLE 1.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Relevant characteristicsa | Ref. or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| MO6–24/O | Clinical isolate; virulent | Laboratory collection |
| OH0701 | MO6–24/O with prx1::nptI; Kmr | 8 |
| OH0505 | MO6–24/O with prx2::nptI; Kmr | 18 |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA 96 thi-1 relA1; plasmid replication | Laboratory collection |
| BL21(DE3) | F− ompT hsdS (rB− mB−) gal (DE3) | Laboratory collection |
| Plasmids | ||
| pGEM-T easy | PCR product cloning vector; Apr | Promega |
| pRSET A | Protein expression vector; Apr | Invitrogen |
| pHIS-parallel1 | Protein expression vector; Apr | 11 |
| pWK0704 | pRSET A with prx1; Apr | 8 |
| pWK0802 | pRSET A with ahpF; Apr | 8 |
| pOH046 | pRSET A with prx2; Apr | This study |
| pOH054 | pRSET A with trxA; Apr | This study |
| pOH053 | pRSET A with trxB; Apr | This study |
| pBANG1105 | pHIS-parallel1 with prx1; Apr | This study |
| pBANG1106 | pHIS-parallel1 with prx2; Apr | This study |
| pBANG1104 | pHIS-parallel1 with trxA; Apr | This study |
| pBANG1016 | pHIS-parallel1 with ahpFNTD; Apr | This study |
| pOH049 | pRSET A with the mutant prx2 encoding Prx2-C50S; Apr | This study |
| pOH050 | pRSET A with the mutant prx2 encoding Prx2-C171S; Apr | This study |
a Apr, ampicillin resistant; Kmr, kanamycin resistant.