TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Oligonucleotide sequence (5′ → 3′)a,b	Use
For expression of proteins
HIS-PRX1 F	CTCGAGATGATTAACACTACTATCAAACCA	Amplification of prx1
HIS-PRX1 R	AAGCTTTTAGATTTTGCCAACTAGGTC
HIS-AHPF F	CTCGAGATGCTAGACCAAGCGATC	Amplification of ahpF
HIS-AHPF R	AAGCTTTTAGCCTTGCTTACGAATCAA
HIS-PRX2 F	GGATCCATGGTACTAGTAGGTCGTC	Amplification of prx2
HIS-PRX2 R	GAATTCTTATTTTTTAAGGTCAGCTG
HIS-TRXA F	GAATTCTTATAGGTTCGCATCCAAAAAC	Amplification of trxA
HIS-TRXA R	GGATCCATGAGTGACAAGATTTTGC
HIS-TRXB F	GGATCCATGAGCGATATGAAACACAG	Amplification of trxB
HIS-TRXB R	GAATTCTTATTTGTCGTTCAACGAGTCTAGG
PRX1-pHIS F	CCATGGCTATGATTAACACTACTATCAAACCATT	Amplification of prx1
PRX1-pHIS R	CTCGAGTTAGATTTTGCCAACTAGGTCTAGTG
PRX2-pHIS F	AACCATGGTACTAGTAGGTCGTCAAGCC	Amplification of prx2
PRX2-pHIS R	CTCGAGTTATTTTTTAAGGTCAGCTGCGTGC
TRXA-pHIS F	CATATGAGTGACAAGATTTTGCAGCTGTC	Amplification of trxA
TRXA-pHIS R	CTCGAGTTATAGGTTCGCATCCAA
HIS-AHPFNTD F	CCATGGCTATGCTAGACCAAGCGATC	Amplification of ahpFNTD
HIS-AHPFNTD R	AAGCTTTTAGCTTGCCGCCGCTTT
For mutagenesis
PRX2-C50S F	CCACTAGACTTCACTTTCGTTAGCCCATCTGAAC	Construction of Prx2-C50S
PRX2-C50S R	GTTCAGATGGGCTAACGAAAGTGAAGTCTAGTGG
PRX2-C171S F	CAGAAGCACGGCGAAGTAAGTCCTGCTCAATG	Construction of Prx2-C171S
PRX2-C171S R	CATTGAGCAGGACTTACTTCGCCGTGCTTCTG
For Northern blot analysis
PRX1001 F	AACTTTGGTGTAATGCGCCC	PRX1P DNA probe
PRX1001 R	CTAGCGTTTGTTCGCCTTCTT
PRX2002 F	ATGGTACTAGTAGGTCGTC	PRX2P DNA probe
PRX2002 R	TTATTTTTTAAGGTCAGCTG

^a The oligonucleotides were designed using the V. vulnificus MO6–24/O genomic sequence (GenBank accession number CP002469 and CP002470, www.ncbi.nlm.nih.gov).

^b Regions of oligonucleotides not complementary to the corresponding templates are underlined.