TABLE 3.
Kinetic parameters of Prx2 and Prx1 with hydrogen peroxide
Enzyme | Reductant | kcat | KmHOOH | Kmreductant | kcat/KmHOOH | kcat/Kmreductant |
---|---|---|---|---|---|---|
s−1 | μma | m−1 s−1 | ||||
Prx2 | TrxA | 0.098 | 1.1 ± 0.01 | 7.9 ± 0.6 | 8.8 × 104 | 1.2 × 104 |
Prx1 | AhpFNTD | 2.9 | 32.6 ± 0.6 | 8.7 ± 0.4 | 8.8 × 104 | 3.3 × 105 |
a ± represent the S.E.