FIGURE 3.

Overexpression of NRSF and co-repressor factors repress CART transcription. A–C, HeLa cells were transfected with the indicated plasmids. Forty-eight hours later, the total RNA was extracted and the mRNA levels of CART (A), the indicated factors (B) and NRSF (C) were measured by qPCR. Data are mean ± S.D. (n = 3). *, p < 0.05. NC means the empty control vector. D, NRSF-depleted HeLa cells were prepared with the lentivirus-mediated RNAi as described under “Experimental Procedures.” These cells were transfected with the indicated plasmid or control empty vector. Forty-eight hours later, the CART mRNA levels were measured by qPCR. Data are mean ± S.D. (n = 3). *, p < 0.05.