FIGURE 4.

Both pNRSE and iNRSE are required for efficient repression of CART expression. A, schematic diagram of the luciferase reporter plasmids that include the promoter and/or intron 1 of human CART gene. B and C, the indicated reporter plasmids along with the pRL-CMV vector were transfected into SK-N-SH (B) or HeLa (C) cells, which express relative lower or higher levels of endogenous NRSF, respectively. The relative luciferase activities are normalized by the activity of the pRL-CMV control. D, schematic diagram of reporter gene constructs that include pNRSE and/or iNRSE elements of the CART gene. Mutations were introduced into the NRSE elements. E and F, the indicated reporter plasmids together with pRL-CMV were transfected into SK-N-SH (E) or HeLa (F) cells and the luciferase activity was measured. Data in B and C, E and F are mean ± S.D. (n = 3).