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. 2012 Oct 18;287(51):42574–42587. doi: 10.1074/jbc.M112.376590

FIGURE 5.

FIGURE 5.

The NRSF-recruited co-repressor protein factors are involved in pNRSE and iNRSE-driven CART repression. A and B, the P-Luc-tI (A) and tP-Luc-I (B) luciferase reporter plasmids described in Fig. 4A (e and d, respectively) were transfected into HeLa cells together with the pRL-CMV control luciferase plasmid, the indicated siRNA against NRSF, CoREST, HDAC1, HDAC2, mSin3A, mSin3B, or the control siRNA (NC). Seventy-two hours post-transfection, the P-Luc-tI and tP-Luc-I luciferase activities were measured and normalized. Data are mean ± S.D. (n = 3). *, p < 0.05. C and D, the P-Luc-tI (A) and tP-Luc-I (B) luciferase reporter plasmids were transfected into HeLa cells together with the pRL-CMV control luciferase plasmid, the indicated overexpression plasmids. NC means the empty vector. Forty-eight hours post-transfection, the P-Luc-tI and tP-Luc-I luciferase activities were measured and normalized. Data are mean ± S.D. (n = 3). *, p < 0.05.