FIGURE 1.

Resveratrol decreases the cell proliferation and induces necrotic cell death of C17.2 NPCs. NPCs were seeded into 96-well plates (1 × 10⁴ cells/ml) and cultured for 24 h. Cells were treated with the indicated concentrations of resveratrol for 12, 24, or 48 h. A, NPCs proliferation was determined using an MTT assay. The higher concentrations of resveratrol inhibited NPCs proliferation. Values are the mean ± S.E. (n = 8). B and C, BrdU immunoreactive cells were counted and values are the mean ± SEs (n = 4). **, p < 0.01 versus vehicle (ANOVA with Fisher's PLSD procedure). D, NPCs were exposed to resveratrol (1, 10, 20, and 50 μm) for 24 h and 10 or 50 μm of Hoechst 33342 and PI were added for 10 min. The Hoechst 33342- and PI-stained cells are bright blue and red, respectively. Scale bar = 100 μm. E, flow cytometry analysis with PI and Annexin V staining showed that 50 μm resveratrol increased PI-positive intensity indicating necrotic cell death in the NPCs.