FIGURE 2.

AMPK activation suppresses NPCs proliferation. A, NPCs treated with resveratrol for 1 h were subjected to Western blotting for AMPK. Resveratrol did activate AMPK (Thr-172). β-Actin was used as loading controls. A representative blot from three independent experiments that yielded similar results is shown. B, to investigate reduced cell proliferation by resveratrol-mediated AMPK activation, NPCs were pretreated with Compound C (5 μm; an AMPK inhibitor) for 1 h. Cells were then incubated for 24 h with or without the indicated concentrations of resveratrol, and MTT assays were performed. Compound C effectively blocked the reduction of cell proliferation by resveratrol. Values are mean ± S.E. (n = 8). **, p < 0.01 versus vehicle in the absence of inhibitor, ##, p < 0.01 versus each treatment in the absence of inhibitor (ANOVA with Fisher's PLSD procedure).