FIGURE 5.

Resveratrol impairs hippocampal neurogenesis. A, schematic overview of the in vivo experimental procedure. C57BL/6 mice (5-week-old) were intraperitoneal administered vehicle or resveratrol was administered for 14 days. BrdU pulses were given the 3 days before vehicle or resveratrol treatment. B, representative images showing BrdU-positive cells in the dentate gyrus. Scale bar, 100 μm. C, numbers of BrdU-positive cells in the dentate gyrus. Representative hippocampal sections (8–10) were chosen and immunostained with BrdU-specific antibodies. The analysis showed fewer newly generated cells in the dentate gyrus in animals treated with resveratrol. Values are mean ± S.E. (n = 5 mice/group). **, p < 0.01 versus vehicle (ANOVA with Fisher's PLSD procedure). D, to determine the effect of resveratrol on hippocampal neurogenesis, double-labeling immunohistochemistry was performed with primary antibodies against BrdU (green) and mature neuron marker (NeuN; red). Hippocampal sections were immunostained with doublecortin (DCX, immature neuron marker) antibody. Resveratrol impaired neuronal differentiation and hippocampal neurogenesis. Scale bar = 100 μm. E, quantitative analysis of BrdU⁺/NeuN⁺-positive cell numbers and DCX-positive cell numbers in the dentate gyrus. **, p < 0.01 versus vehicle (ANOVA with Fisher's PLSD procedure).