FIGURE 1.

LBR possesses a tudor domain in the N-terminal region. A, schematic illustration of the full-length LBR. The amino acids 1–62 correspond to the tudor domain (green). Blue, red, and purple squares correspond to RS, globular II, and transmembrane regions, respectively. NP represents the whole nucleoplasmic region of LBR. Asterisks indicate the positions of mutated amino acid residues. Numbers indicate the number of amino acid residues from the N terminus. B, alignment of the tudor domain sequences. The amino acid residues of LBR are numbered above the sequences. Green and yellow boxes indicate identical and highly conserved amino acids, respectively. The amino acid residues substituted in this study are indicated by stars. C, comparison of the three-dimensional structure of the tudor and chromo domains. Structural information was obtained from the NCBI Data Bank (Protein Data Bank ID codes for LBR, JMJD2A, 53BP1, and HP1 are 2DIG, 2GFA, 2IG0, and 1Q3L, respectively). The electrostatic potential of the molecular surface was calculated by MacroModel software. Red, blue, and white regions indicate negative, positive, and neutral charges, respectively. The histone peptides recognized by each domain are shown as stick models, and the specific histone codes recognized by the domains are shown at the bottom. Histone code-reading pockets are indicated by yellow circles.