Change of Mash2 expression is similar to TSSC3.
A, top panels, Tssc3 and Mash2 mRNA levels in MASH2-overexpressing TS cells. Bottom panels, TSSC3 and Mash2 protein levels in MASH2-overexpressing TS cells. B, mRNA expression levels were assessed by real-time PCR using cDNA from MASH2-overexpressing TS cells. The mRNA levels were normalized using Gapdh. Results are the mean ± S.D. (error bars) from three independent experiments (*, p < 0.01). C, these panels show TSSC3 and MASH2 protein levels in MASH2 knockdown TS cells. D, Mash2 and Tssc3 mRNA expression levels were assessed by real-time PCR using cDNA from MASH2 knockdown TS cells. mRNA levels were normalized using Gapdh. Results are the mean ± S.D. from three independent experiments (*, p < 0.01; **, p < 0.05).