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. 2012 Oct 15;287(51):42685–42694. doi: 10.1074/jbc.M112.388777

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — TSSC3 up-regulation of Mash2 as a transcription factor. A, luciferase activity with Ad-TSSC3, Ad-MASH2, Ad-TSSC3, and MASH2. A reporter gene possessing the E-box was constructed. Luciferase activity values were normalized using β-galactosidase activity values. Results are the mean ± S.D. (error bars) from three independent experiments (*, p < 0.01). B, alteration in luciferase activity of E-box reporter by knockdown of TSSC3 or MASH2 by siRNA in TSSC3-overexpressing TS cells. Results are the mean ± S.D. from three independent experiments (*, p < 0.01). C, alteration of cell growth by overexpression of TSSC3 or MASH2 in TS cells is shown. D, alteration of cell growth by knockdown of TSSC3 or MASH2 in TS cells is shown.