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. 2012 Oct 26;287(51):42784–42794. doi: 10.1074/jbc.M112.410936

FIGURE 4.

FIGURE 4.

Effect of transient expression of Rab26 mutants and siRNA-mediated knockdown of Rab26 on α2B-AR-mediated ERK1/2 activation. A, effect of Rab26 on α2B-AR-mediated ERK1/2 activation. HEK293 cells stably expressing α2B-AR were transfected with the pEGFP-C1 vector (Ctrl), GFP-tagged wild-type Rab26 (WT), or individual Rab26 mutant. The cells were stimulated with UK14304 at a concentration of 1 μm for 5 min, and ERK1/2 activation was determined by immunoblotting using phospho-specific ERK1/2 antibodies. B, quantitative data shown in A. C, effect of siRNA-mediated knockdown of Rab26 on α2B-AR-mediated ERK1/2 activation. HEK293 cells stably expressing α2B-AR were transfected with control siRNA (Ctrl) or Rab26 siRNA and stimulated with UK14304 as described above. NT, nontransfection. D, quantitative data shown in C. In A and C, top panels, representative blots showing ERK1/2 activation; middle panels, total ERK1/2 expression; bottom panels, Rab26 expression. In B and D, the data are expressed as percentages of ERK1/2 activation obtained from control cells and are presented as the means ± S.E. of three experiments. *, p < 0.05 versus control.