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. 2012 Oct 29;287(51):42867–42880. doi: 10.1074/jbc.M112.407072

FIGURE 7.

FIGURE 7.

Disruption of di-leucine motif delays trafficking to early and late endosomes/lysosomes and retrotransport to TGN following endocytosis. All line graphs represent mean values of co-localizing pixels ±S.E. (**, p < 0.001; *, p < 0.05, one-way ANOVA), scale bar, 5 μm. All three stainings show a clear retention of BACE1LLAA and BACE1LLAA/KR at the cell membrane. A, representative photomicrographs of BACE1WT, BACE1KR, BACE1LLAA, and BACE1LLAA/KR after 15, 30, and 180 min of internalization and stained with the early endosomes marker EEA1. B, line graph shows accumulation of BACE1LLAA and BACE1LLAA/KR in the early endosomes after 30 and 180 min internalization, respectively. C, representative photomicrographs of BACE1WT, BACE1KR, BACE1LLAA, and BACE1LLAA/KR after 15, 30, and 180 min of internalization and stained with the late endosomes/lysosomes marker LAMP2. D, line graph shows accumulation of BACE1LLAA and BACE1LLAA/KR in the late endosomes and lysosomes after 180 min of internalization. E, representative photomicrographs of BACE1WT, BACE1KR, BACE1LLAA, and BACE1LLAA/KR after 15, 30, and 180 min of internalization and stained with the trans-Golgi network marker TGN38. F, line graph shows delayed transport of BACE1LLAA and BACE1LLAA/KR to the trans-Golgi network.