FIGURE 2.

BRAP interacts with both catalytically active and inactive USP4 and 15 but not USP11 in a cellular environment. HEK293T cells coexpressing myc-tagged BRAP and GFP-tagged USP4, -11, -15, GFP-BRAP- or GFP were lysed in RIPA buffer and subjected to immunoprecipitation (IP) with anti-GFP antibodies and probed with anti-myc and anti-GFP. Bound proteins were analyzed alongside 5% of input sample. IB, immunoblot. Arrows indicate higher molecular weight forms of BRAP. The bottom panel shows a higher intensity representation of input lanes. U, USP; CS, catalytically inactive mutant.