TABLE 1.
The USP15 and USP4 DUSP-UBL mediates binding to the BRAP C-terminal coiled coil region
Fragments of USP4, USP15, USP11, and BRAP were inserted into bait (USPs) and prey (BRAP) Y2H vectors and tested for interaction by directed Y2H. +, interaction seen with HIS3 reporter only; ++, interaction shown with HIS3 and ADE2 reporters. ND, not determined. Cat. domain, catalytic domain; ΔNT, N-terminal deletion mutant; CC, coiled coil.
| BAIT (USPs) | PREY (BRAP) |
||
|---|---|---|---|
| Full-length | ΔNT (141–592) | CC (393–592) | |
| Full-length USP15 | + | ND | ND |
| USP4 | − | ND | ND |
| USP11 | − | ND | ND |
| DUSP-UBL USP15 | − | ++ | + |
| USP4 | − | ++ | + |
| USP11 | − | ++ | − |
| UBL USP15 | − | − | − |
| USP4 | − | − | − |
| USP11 | − | − | − |
| DUSP USP15 | − | − | − |
| USP4 | − | − | − |
| Cat. domain USP15 | − | − | − |
| USP4 | − | − | − |