Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 29;287(51):43030–43041. doi: 10.1074/jbc.M112.380964

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Selected NMR ¹⁵N-¹H chemical shift perturbations of CaM and CaM-N due to Orai1-CMBD. A, titration behavior for the amide resonance of Ile¹³⁰ (located in the C-lobe) of the full-length CaM is shown upon addition of Orai1-CMBD at protein to peptide ratios of 1:0, 1:0.5, 1:1, 1:1.5, and 1:2.0. B, titration behavior for the amide resonance of Ala⁵⁷ (located in the N-lobe) in the full-length CaM titrated as in A. C, behavior of the amide resonance of Ala⁵⁷ in isolated CaM-N is shown upon titration of Orai-CMBD at protein to peptide ratios of 1:0, 1:0.5, and 1:1. The panels are aligned for comparison with Ala⁵⁷ in full-length CaM. Note that the N-terminal Ala⁵⁷ amide resonance is not significantly perturbed until the peptide:protein ratio exceeds 1.