TABLE 2.
Single-channel characteristics of KvLm PM reconstituted in monoolein bilayers
Single-channel current recordings in which only one channel was open at any given time were analyzed to determine the channel dwell times in the open and closed states. The experimental conditions were 0.5 m KCl, 10 mm Hepes, pH 7.4, recorded at 100 mV, and 27 mm KCl, 10 mm Hepes, pH 7.4, recorded at 150 mV. For τ1 closed and τ2 closed, the relative area under the fitted curve is indicated in parentheses and represents the frequency of occurrence of each state. The results are cumulative from three different experiments; error indicates S.E.
| Experimental condition | 0.5 m KCl | 27 mm KCl |
|---|---|---|
| Conductance (pS) | 68 ± 9 | 4 ± 2 |
| Open occupancy | 0.1 ± 0.1 | 0.3 ± 0.2 |
| Closed occupancy | 0.9 ± 0.1 | 0.7 ± 0.2 |
| τopen (ms) | 1.3 ± 0.3 | 12 ± 5 |
| τ1 closed (ms) | 1.8 ± 0.5 (0.62) | 13 ±5 (0.66) |
| τ2 closed (ms) | 350 ± 40 (0.38) | 113 ± 50 (0.34) |
| Burst length (ms) | 14 ± 3 | 70 ± 10 |
| Total openings analyzed | 20,200 | 35,202 |