FIGURE 1.

Expression levels of Gp96 are not affected by L. monocytogenes cellular infection. A, levels of gp96 expression were assessed by real time RT-PCR in Caco-2 and J774 non-infected (NI) or infected with L. monocytogenes WT or Δvip for different periods of time. Represented values are relative to the expression of gapdh in the same conditions. Error bars represent S.D. B, Caco-2, L2071, and J774 cells were left non-infected or were infected with L. monocytogenes WT or Δvip for 1, 2, and 4 h. Cells were lysed at different time points, and levels of Gp96 were assessed by Western blot. Actin was used as loading control. C, for each condition, expression levels of Gp96 relative to the expression of actin were quantified. p.i., post-infection.