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. 2012 Dec 12;7(12):e51271. doi: 10.1371/journal.pone.0051271

Table 1. Information on primers used for real-time qPCR.

Gene Accession number Sequence 5′→3′ Fragment size (pb) Efficiency (%) Correlation (R2)
IMPACT ENSOCUT00000013903 GCGTCTTCTTCACCTCATGG 116 104.8 0.99
TGTTTCTTGGCACAGTTGTTGA
SMARCA2 ENSOCUT00000006331 AATCCGCAACCACAAGTAAC 113 103.1 0.99
GAACACTGACTGTAAGACGAT
EMP1 ENSOCUT00000021095 AATGTTGGTGTTACTGGCTG 110 100.2 0.98
GATGCGTTAATAGAGTCTGAA
SCGB1A1 ENSOCUT00000014246 CCAGTTACGAGACATCCCTGA 155 93 0.99
CATACACAGTGGGCTCTTCACT
DPY30 ENSOCUT00000021095 GCAGAGAACCCTCATTCTGAG 148 98.4 0.99
CGCACAACTGTCTGATCCTGGT
CALC ENSOCUT00000003074 GCTAGAGACTGAGGGCTCCA 124 90.8 0.99
CACGAAGTTGCTCTTCACCA
H2AFZ AF030235 AGAGCCGGCTGCCAGTTCC 85 98.8 1
CAGTCGCGCCCACACGTCC
GAPDH L23961 GTTCTTCTCGTGCAG 144 93.1 1
ATGGATCATTGATGGCGACAACAT

H2AFZ: H2A histone family member Z [35]; GAPDH: glyceraldehyde-3-phosphate dehydrogenase [36]; SMARCA2: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; EMP1: Epithelial membrane protein 1; CALC: calcitonin gene-related peptide variant 1; SCGB1A1: secretoglobin family 1A member 1).