Table 1. Information on primers used for real-time qPCR.
Gene | Accession number | Sequence 5′→3′ | Fragment size (pb) | Efficiency (%) | Correlation (R2) |
IMPACT | ENSOCUT00000013903 | GCGTCTTCTTCACCTCATGG | 116 | 104.8 | 0.99 |
TGTTTCTTGGCACAGTTGTTGA | |||||
SMARCA2 | ENSOCUT00000006331 | AATCCGCAACCACAAGTAAC | 113 | 103.1 | 0.99 |
GAACACTGACTGTAAGACGAT | |||||
EMP1 | ENSOCUT00000021095 | AATGTTGGTGTTACTGGCTG | 110 | 100.2 | 0.98 |
GATGCGTTAATAGAGTCTGAA | |||||
SCGB1A1 | ENSOCUT00000014246 | CCAGTTACGAGACATCCCTGA | 155 | 93 | 0.99 |
CATACACAGTGGGCTCTTCACT | |||||
DPY30 | ENSOCUT00000021095 | GCAGAGAACCCTCATTCTGAG | 148 | 98.4 | 0.99 |
CGCACAACTGTCTGATCCTGGT | |||||
CALC | ENSOCUT00000003074 | GCTAGAGACTGAGGGCTCCA | 124 | 90.8 | 0.99 |
CACGAAGTTGCTCTTCACCA | |||||
H2AFZ | AF030235 | AGAGCCGGCTGCCAGTTCC | 85 | 98.8 | 1 |
CAGTCGCGCCCACACGTCC | |||||
GAPDH | L23961 | GTTCTTCTCGTGCAG | 144 | 93.1 | 1 |
ATGGATCATTGATGGCGACAACAT |
H2AFZ: H2A histone family member Z [35]; GAPDH: glyceraldehyde-3-phosphate dehydrogenase [36]; SMARCA2: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; EMP1: Epithelial membrane protein 1; CALC: calcitonin gene-related peptide variant 1; SCGB1A1: secretoglobin family 1A member 1).