Table 5. Real-time quantitative PCR assay for six randomly selected genes.

	Relative expression (a.u.)		Fold change
Gene	Fertilised embryos	Parthenote embryos	RT-qPCR	Microarray
IMPACT	0.82±0.16a	0.004±0.21b	7.68	4.58
DPY30	1.24±0.14a	0.27±0.18b	2.20	3.23
CALC	0.56±0.04a	0.14±0.05b	2.00	7.54
SCGB1A1	0.04±0.22a	1.25±0.25b	−4.96	−9.51
EMP1	0.48±1.99a	8.37±1.99b	−4.12	−6.07
SMARCA2	0.16±0.51a	1.76±0.51b	−3.45	−6.21

SMARCA2: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; EMP1: Epithelial membrane protein 1; CALC: calcitonin gene-related peptide variant 1; SCGB1A1: secretoglobin family 1A member 1). Relative expression values are shown in arbitrary units (a.u), expressed by the mean value ± standard error means. Letters with different superscripts are significantly different (P<0.05). RT-qPCR fold changes were obtained by calculation of log2 transformed ratio of relative expression for each gene. Microarray fold changes were obtained by log2 transformed probe intensities for each gene.