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Sumoylated proteins are enriched in the region of the redundant nuclear envelope in human sperm. (A1–A3) SUMO2/3 (green) is significantly colocalized with an antibody against the nuclear pore complex (NPC, red), which serves as a marker of the redundant nuclear envelope (RNE) in human sperm. The scale bar is 5 µm. (B) Immunogold labeling of human sperm with an anti-SUMO2/3 antibody; positive staining in the neck area is highlighted by dashed circles. M, mitochondria; C, centriole.
