Figure 3.

(A) Inhibition of rhuZP3β-triggered the acrosome reaction in human sperm by adjudin. Sperm were capacitated in the HTF medium for 5 h, washed, resuspended in the fresh HTF medium and exposed to different concentrations of adjudin (10 nM–10 µM) for 15 min and then the acrosome reactions were triggered with rhuZP3β (20 µg/ml) for 15 min. Results are expressed as the mean ± SEM (n = 3). a when compared with control, P < 0.001; b compared with a (rhuZP3β alone), P < 0.01 to <0.001. c versus b, P < 0.05; d versus c, P < 0.05. (B) Inhibition of progesterone-induced acrosome reaction in human sperm by adjudin. Sperm were incubated in the HTF medium under capacitating conditions for 5 h to induce capacitation, washed, resuspended in the HTF containing different concentrations of adjudin for 15 min and then elicited with 15 μM P₄ for 15 min before evaluation of the acrosome reaction. Results are expressed as the mean ± SEM (n = 4). a when compared with control, P < 0.001; b when compared with progesterone, P < 0.01 to <0.001.