Figure 5.

Effect of adjudin on human sperm protein phosphorylation during capacitation. The western blotting and the quantitative analysis of signal intensity of protein phosphorylation were performed as described in the section Materials and methods. (A) tyrosine, (B) serine and (C) threonine phosphorylation. Each experiment was repeated at least three times. The bands indicated by arrows were further analyzed by densitometry. Their phosphorylated levels (D–F) were expressed by the ratio of the gray intensity of bands compared with loading controls, β-tubulin (mean ± SEM, n = 3). The results indicated that 10 and 100 µM adjudin were not capable of inhibiting the increase in protein tyrosine phosphorylation compared with the control, P > 0.05, but did inhibit serine phosphorylation at 70 kDa protein, and threonine phosphorylation at 60, 72, 95, 110 and 130 kDa proteins, when compared with the control, *P < 0.05.