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. 2012 Oct 31;28(1):47–59. doi: 10.1093/humrep/des384

Figure 6.

Figure 6

Inhibition by adjudin (A), CFTRinh-172 (B) and bicuculline (C) on a combination of forskolin- and pentoxifylline- or progesterone-induced capacitation in human sperm. Sperm were preincubated with or without a combination of forskolin (10 μM) and pentoxifylline (3.6 µM) or progesterone (15 μM) alone for 5 h in the presence of or in the absence of 10 μM adjudin (A), 1 μM CFTRinh-172 (B) or 10 μM bicuculline (C) in the HTF medium under capacitating conditions, samples were washed and then incubated for 30 min before evaluation of sperm capacitation (‘B’ + ‘AR’ patterns). The results are expressed as the mean ± SEM of five different experiments (n = 5). For each group, letters above bars indicate significant differences. a, when compared with CTL, P < 0.001; b versus a, P < 0.01; c versus b, P < 0.01; a versus c, P < 0.001. CTL, control; CFTR, cystic fibrosis transmembrane conductance regulator; P4, progesterone.