Table II.

Inhibition of human sperm penetration of zona-free hamster eggs by adjudin.

Treatment	Number of eggs examined	Number of eggs penetrated	Penetration index	Percentage of penetration
Control	65	46	2.8	70.8
Adjudin (10µM)	86	24	1.2	27.9*
Progesterone (P4, 15 µM)	44	36	3.0	81.0

Sperm were preincubated with or without adjudin 10 µM in HTF for 5 h, washed, added separately to BWW medium containing 15–20 zone-free hamster oocytes and then co-incubated for 3 h at 37°C under 5% CO₂ in air. Results are expressed as mean ± SEM. These data were represented in five different experiments (n = 5); adjudin significantly different from controls used. Paired samples test (*P < 0.001).