Table 2.
Cytochrome P450 activities of various enzyme source preparations with three luminescent substrates and one fluorescent substrate using Marin and CQ-1 larvae
| P450 activity (pmolluciferin/min/µ g protein) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Enzyme sourcea |
Luciferin-BE |
Luciferin-H |
Luciferin-Me |
||||||
| Marin | CQ-1 | AR | Marin | CQ-1 | AR | Marin | CQ-1 | AR | |
| Mgutb | 990 (32.6) | 1,180 (57.9) | 0.84 | 209 (12.4) | 128 (14.0) | 1.6 | 495 (43.4) | 394 (37.2) | 1.3 |
| Hgutc | 371 (40.2) | 426 (110) | 0.87 | 68.9 (11.1)d,e | 46.0 (7.96) | 1.5 | 149 (20.1) | 120 (11.4) | 1.2 |
| Hcaecaec | 117 (12.2) | 1.84 (68.9) | 0.64 | 16.8 (3.21) | 14.4 (6.76) | 1.2 | 49.9 (14.9) | 57.5 (27.8) | 0.87 |
| Hventriculusc | 349 (46.1)d,f | 268 (28.3) | 18.3 | 71.8 (19.8)d,g | 26.2 (9.09) | 2.7 | 213 (4.53)d,h | 93.5 (20.8) | 2.3 |
| Htubulesc | 43.6 (18.3) | 59.6 (16.3) | 0.73 | 25.2 (5.51) | 26.2 (8.73) | 0.96 | 32.1 (9.10) | 33.8 (6.06) | 0.95 |
| S-Hguti | — | — | — | 33.5 (15.1)jk | 24.6 (8.35) | 1.8 | — | — | — |
| Sgut(Lu-H)i | — | — | — | 112 (58.3)j,l | 62.3 (28.3) | 1.8 | — | — | — |
| Sgut(7-EC)i | — | — | — | 1,840m (543)j,n | 1,440m (321) | 1.3 | — | — | — |
| Slive(Lu-H)i | — | — | — | 0.964 (0.348)j,o | 0.760 (0.209) | 1.3 | — | — | — |
| Slive(7-EC)i | — | — | — | 117m (91.5)j,p | 77.4m (52.4) | 1.5 | — | — | — |
Values are mean (SD).
See Table 1.
Activities reported as the average of six replicate wells per substrate from individual mosquito gut microsomal preparations.
Activities reported as the average of at least three homogenates from larvae of different filial generations.
Marin mean significantly higher than CQ-1 by Student's t-test (Sigmastat 2004).
t = 2.91; df = 4; P = 0.04.
t = 3.01; df = 6; P = 0.02.
t = 4.20; df = 6; P = 0.006.
t = 4.82; df = 6; P = 0.003.
Activities reported as the average of n single preparations. See Table 1 for n for Marin preparations, n of CQ-1 = 30, 39, 24, 51, and 115 for S-Hgut, S-Hgut(H)), Sgut(7-Ec), Slive(H), and Slive(7-EC) preparations, respectively.
Marin mean significantly higher than CQ-1 by Mann-Whitney test (Sigmastat 2004).
T = 920 P = 0.03.
T = 1104; P ≤ 0.001.
Pmoles 7-OH coumarin per minute per microgram protein.
T = 626; P = 0.003.
T = 2202; P = 0.004.
T = 11466; P ≤ 0.001.
AR, activity ratio of Marin/CQ-1 for each preparation; –, not tested.