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. 2012 Nov 30;33(1):e00003. doi: 10.1042/BSR20120093

Figure 4. Analysis of second round basic loop mutants.

Figure 4

(A) Expression of the gp64 basic loop mutants following transfection of Sf9 cells. (B) Syncytia formed by the gp64 basic loop mutants in the Sf9 cells following transfection and a transient pH shock. (C) Quantification of rescued virus stocks carrying selected gp64 basic loop mutants determined by Western blotting of relative gp64 level on concentrated virus preparations. The antibody used was B12D5 [66]. (D) Visualization of plaque assays by WT and RR–RR viruses. Monolayers of Sf9 cells were incubated with equivalent doses of virus as determined by the gp64 level and visualized at 2 days post-infection.