Figure 4. Effect of TAK-242 on IRS-1 phosphorylation.

L6 myotubes were incubated with/without TAK-242 (1 μM) for 1 h prior to LPS (100 ng/ml) for 1 h (A) or stearate (400 μM) for 6 h (B). Cells were lysed and equal amounts of total protein from each sample were immunoblotted with specific antibodies against phospho-IRS-1 on Ser¹¹⁰¹ (p-IRS-1 Ser¹¹⁰¹) and total IRS-1. Data are expressed as a ratio of phosphorylated to total protein, in arbitrary units as fold change from control. Representative immunoblots are shown above. Results are means±S.E.M. of two independent experiments performed in triplicate. *P<0.05 against control.