Figure 1. Truncation of lipid-free apoA-I and apoA-I in HDL₃ by chymase digestion.

Lipid-free apoA-I (A) and HDL₃ (B) were treated with (+) or without (−) chymase (0.03 BTEE unit/ml for lipid-free apoA-I and 3.0 BTEE unit/ml for HDL₃) for the indicated times (min). The digested mixtures were analysed by SDS/PAGE followed by WB using anti-apoA-I antibody (Po) and 16-4 mAb, which specifically reacts with truncated apoA-I at the carboxyl side of Phe²²⁵ [8].