Figure 5. Simultaneous digestion of apoA-I by chymase and CPA.
Lipid-free apoA-I was simultaneously digested by 0.05 BTEE unit/ml of chymase and 5 μg/ml of CPA for 0–4 h and analysed by SDS/PAGE followed by WB using anti-apoA-I antibody (Po) and 16-4 mAb. Lipid-free apoA-I digested by only chymase and non-digested lipid-free apoA-I were also analysed as controls.