Table 1. Summary of chymase-digested truncated apoA-I.
| Peak* | m/z† | N-terminal‡ | tapoA-I§ | Mass∥ | PAGE¶ | pI∥ | 2-DE** |
|---|---|---|---|---|---|---|---|
| Lipid-free apoA-I | |||||||
| Intact apoA-I | 28076 | 1- | 28080 | 28 kDa | 5.27 | ||
| Peak 1 | 26454 | − | Δ(230–243) | 26441 | 26 kDa | 5.25 | Spot 1, 2 |
| Peak 2 | 25964 | − | Δ(226–243) | 25980 | 26 kDa | 5.17 | Spot 2, 3 |
| 25964 | − | Δ(1–18) | 25982 | 26 kDa | 5.45 | Spot 6 | |
| Peak 3 | 22374 | 1- | Δ(193–243) | 22368 | 24 kDa | 5.04 | Spot 4, 5 |
| HDL3 | |||||||
| Intact apoA-I | 28079 | − | 28080 | 28 kDa | 5.27 | ||
| Peak 4 | 25980 | − | Δ(1–18) | 25982 | 26 kDa | 5.45 | – |
| Peak 5 | 24271 | 34- | Δ(1–33) | 24272 | 24 kDa | 5.56 | Spot 7, 8 |
*Peak number detected by MALDI–TOF-MS.
†Representative m/z obtained from MALDI–TOF-MS.
‡Detected N-terminal sequence by top-down sequencing.
§Estimated truncated apoA-I.
∥Calculated molecular mass and pI of tapoA-I.
¶Apparent molecular mass obtained from SDS/PAGE.
**Corresponding spot number on 2-DE pattern.