Figure 5.

HaCaT cells from different sources exhibit differential growth in co-culture. HaCaT cells used to generate results shown in figures 1 to 4 were obtained from an early passage source (39) whereas HaCaT-CBL (Click Beetle Luciferase) were obtained from Dr. Stewart’s laboratory where they are used to study transformative effects of tumor microenvironment on epithelial cells (48). The different HaCaT cells were plated at 500 cells per 60 mm dish with fibroblasts (DC-1 or N-2). Error bars represent standard error of results from triplicate plates.