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. Author manuscript; available in PMC: 2014 Feb 8.
Published in final edited form as: Oncogene. 2012 Sep 10;32(32):3765–3781. doi: 10.1038/onc.2012.388

Figure 11.

Figure 11

Figure 11

Figure 11

The sensitivity of A549 cells to chemotherapeutic agents is increased by inhibition of the PI3K-PKB/Akt pathway

A549 cells were seeded and grown in 96-well plates for 24 h before being subjected to serum depletion (in 0.1% FBS) for 16 h.

A) The A549 cells were then pretreated for 8 h with LY294002, or LY294002 plus CT99021, before they were challenged with various doses of acrolein, cisplatin or chlorambucil for 48 h and cytotoxicity measured using the MTT assay.

B) The A549 cells were pretreated with MK-2206, or MK-2206 plus CT99021, for 8 h before being exposed to acrolein, cisplatin or chlorambucil for 48 h and MTT cytotoxicity testing performed.

C) An MTT curve for A549 cells treated for 48 h with different doses of LY294002, MK-2206 or CT99021 in medium containing 0.1% FBS is shown as a control.