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. 2012 Dec 14;7(12):e51952. doi: 10.1371/journal.pone.0051952

Figure 1. miRNA-based regulation of TetR-KRAB control of transgene expression.

Figure 1

(a) Schematic representation of the miRNA-TetR-KRAB regulatory and reporter cassettes. (b) In the absence of tissue-specific miRNA, the repressor is translated and is free to bind the tetO operator, thus preventing transgene expression. However in the presence of a tetracyclin analog (Doxycycline) the repressor does not bind and transcription is activated.(c) In targeted cells tissue-specific miRNA binds to its complementary target and results in degradation of the TetR-KRAB mRNA. As a result, transgene expression occurs.