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. 2012 Dec 14;7(12):e51952. doi: 10.1371/journal.pone.0051952

Figure 4. Lentiviral vectors used to target transgene expression to macrophage-derived cells.

Figure 4

(a) Schematic diagram of the lentivirus vectors used to target cells of immune lineage. (b) miR-142 expression levels in Huh7, 293T and NR8383 cells detected by RT-qPCR. Three independent experiments were performed in duplicate. * Statistically significant differences P  = 0.043 (two-tailed, unpaired Student’s t-test). (c) & (d) Huh7, 293T and NR8383 cells were transduced with two different lentiviral vectors carrying the TetR-KRAB sequence follow by 4 copies of miR-142 target (c) or miR-122 (d). After 10 days of culture with or without doxycycline, transduced cells were analyzed for GFP expression by FACS. Results are expressed as a percentage of mean fluorescence intensity (MFI). In TetR-Krab system, the expression of GFP is optimal in the presence of doxycycline, corresponding to 100% of the MFI. The raw data corresponding to 100% are indicated above each histogram bar.Data shown are mean and error bars indicate the SD of 3 independent experiments performed in triplicate.