Figure 2. Puromycin stimulates the production of truncated polypeptides in a dose-dependent manner.

A. 293-K^b cells were radiolabeled with [³⁵S]-Met for 10 minutes in the presence of a linear range of puromycin concentrations from 0 to 20 µM. Radiolabeled polypeptides were visualized as described for Fig. 1B. In the later panels, we analyze the [³⁵S] signals from the four regions indicated to the right of the gel. B. PhosphorImager signal intensities (arbitrary units) from selected lanes in A. The left side of the graph corresponds to the top of the gel while the right side of the graph corresponds to the bottom of the gel at the dye front. The highlighted regions correspond to the parts of the gel indicated in A. C. The effects of puromycin concentration on [³⁵S] signal for each of the highlighted gel regions in A and B. Results are representative of three independent experiments.