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. 2012 Dec 14;7(12):e51968. doi: 10.1371/journal.pone.0051968

Figure 5. MHC class I-peptide complex recovery assay using a fluorescent reporter encoding antigenic peptides.

Figure 5

A. Schematic of the modified NP-SIINFEKL-eGFP reporter (adapted from [2]) containing eight additional tandem repeats of SIINFEKL (nine total) and its five flanking amino acids from the native ovalbumin sequence, NP-[SIINFEKL]9-eGFP (Tandem Repeat x9 or TRx9). B. Validation of MHC I peptide stripping and recovery in TRx9-expressing cells. Biexponential scatter plots show single cell profiles of the mean fluorescence intensity (MFI) for eGFP on the x-axis and 25-D1.16 on the y-axis. Plots show fluorescence profiles immediately pre- (left) and post- (middle) peptide stripping, and after a 4 hour recovery (right).