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. 2012 Dec 14;7(12):e51973. doi: 10.1371/journal.pone.0051973

Figure 3. RABD2a protein is stable when expressed from 2A constructs.

Figure 3

Immunoblot analysis of protein extracts from Arabidopsis protoplasts transiently transfected with non-tagged RABD2a (positive control), CFP (negative control), CFP-2A-RABD2a, SP-CFP-2A-RABD2a, GS-CFP-2A-RABD2a and CFPGUS-2A-RABD2a using anti-RABD2 antiserum. Native RABD (red arrow, N), overexpressed RABD2a (black arrow, O), 2A derived RABD2a (black arrow, *) and non-cleaved 2A polyprotein (**) are indicated. Low expression and high cleavage efficiency of CFPGUS-2A-RABD2a construct makes no detectable full length CFPGUS-2A-RABD2a band, and only faint band of released RABD2a (*) visible at this exposure time.