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. 2012 Dec 14;7(12):e51973. doi: 10.1371/journal.pone.0051973

Figure 6. Effect of mutant forms of small GTPases on trafficking of HACAH1-2A.

Figure 6

Immunoblot analysis using anti-HA antiserum of protein extracts from Arabidopsis protoplasts (A, B and C) and Nicotiana benthamiana leaves (D and E) expressing HACAH1-2A-RABD2a (wt) or mutant (N121I) form (A and D); HACAH1-2A-SAR1 (wt) or mutant (H74L) form (B); HACAH1-2A-ARF1 (wt) or mutant (T31N) and (Q71L) forms (C and E). Full length non-cleaved HACAH1-2A-RABD2A/SAR1/ARF1 (**), 2A cleaved HACAH1-2A (*), Endo H resistant (black triangle) and susceptible (white triangle) forms of HACAH1-2A are indicated. (F and G) The ratio of resistant (black triangle) to total HACAH1-2A (white+black triangle) cleaved forms (*) was calculated for Arabidopsis protoplasts (F) and N. benthamiana leaves (G). Error bars show standard error (n = 4, ** = p<0.01, *** = p<0.001).