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. 2012 Dec 14;7(12):e51825. doi: 10.1371/journal.pone.0051825

Figure 5. Optimization of siRNA treatment in the tested cell lines. A.

Figure 5

The effect of GAPDH knockdown on the proliferative fraction of each cell line was determined. As expected, GAPDH knockdown inhibits proliferation in all cell types. B. Time course of reduction in TRPM8 RNA content by the indicated TRPM8 siRNA treatment. TRPM8 message was determined at the indicated times after transfection. Relative abundance is referred to the amount of mRNA at time 0 and corrected for the expression of transferrin receptor as control. C. TRPM8 protein knockdown by TRPM8.3 and TRPM8.4 siRNA 48 h after transfection. Actin was used as loading control, and the densitometric quantification relative to control siRNA is presented on the right bar diagram.