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. 2012 Dec 14;7(12):e51825. doi: 10.1371/journal.pone.0051825

Figure 6. siRNA reduces the proliferative fraction of prostatic cancer cells. A.

Figure 6

. Representative cell cycle histogram of DU145 cells transfected with control (top) or hTRPM8 siRNA (bottom). The proliferative fraction (S/G2/M) is clearly reduced after TRPM8 knockdown. B. Proliferative fraction (%) of cells transfected with control or anti TRPM8 siRNA (sequences 3 and 4). The bars represent average of 3–5 experiments. C. BCTC maintains its inhibitory effects after silencing of TRPM8. Normalized growth of DU145 cells in the presence of 10 µM BCTC, after transfection of the cells with control (white column) or TRPM8.4 siRNA (black column). Growth in the absence of BCTC represents 100%.