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The binding of Y-142 to a linear or conformational epitope was tested using Western blot. sHB-EGF (A) or ARG (B) was prepared in reducing or non-reducing conditions with or without dithiothreitol, respectively. The sHB-EGF was probed with an anti-HB-EGF polyclonal antibody and with Y-142. The ARG was probed with anti-ARG polyclonal antibody and with Y-142.
