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. 2012 Dec 14;7(12):e51964. doi: 10.1371/journal.pone.0051964

Figure 6. Recognition of F115, G119, Y123, G137, G140, and R142 by Y-142.

Figure 6

Epitope mapping of Y-142 was performed using alanine scanning. Each mutant proHB-EGF expression plasmid was transfected into SW480 cells. The binding activity of Y-142 to the cells was measured in a cell ELISA. The expression level of mutant proHB-EGF was normalized with the binding of anti-HB-EGF polyclonal antibody by each mutant. The binding of Y-142 to mutant proHB-EGF was calculated as a percentage of the “control” binding of Y-142 to wild-type proHB-EGF. Data points represent the mean + SD of values acquired in triplicate.