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. 2012 Dec 14;7(12):e52190. doi: 10.1371/journal.pone.0052190

Figure 1. Tac-Kremen1 co-localizes with endocytic proteins.

Figure 1

HeLa cells were transfected with (A) Tac alone or (B) Tac-Kremen1. Cells were fixed and permeabilized and stained with mouse monoclonal anti-Tac and Rabbit anti-AP-2 β2 -subunit GD/1. Inset in (B) is zoomed to confirm the co-localization of AP-2 and Tac-Kremen1. HeLa cells were co-transfected with (C) clathrin-LC-eYFP and Tac-Kremen1 or (D) caveolin1-eGFP and Tac-Kremen1. Cells were fixed and permeabilized and incubated with mouse monoclonal anti-Tac antibody. Scale bar 10 µm.