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. 2012 Dec 14;7(12):e52293. doi: 10.1371/journal.pone.0052293

Figure 3. Alignment of predicted 3′ UTR sequences.

Figure 3

TGA/TAA stop codons and the polyadenylation signals, AATAAA, are boxed. The UTRs are determined from genomic DNA and mRNA. Nim-UTR was determined by alignment of N. inaurata madagascariensis flagelliform spidroin gene (GenBank accession no. AF218623S2) and N. clavipes flagelliform (Flag) mRNA (GenBank accession no. AF027973); Lh-UTR 1 was determined by alignment of L. hesperus major ampullate spidroin 1 (MaSp1) gene (GenBank accession no. EF595246) and mRNA (GenBank accession no. DQ409057); Lh-UTR 2 was determined by alignment of L. hesperus major ampullate spidroin 2 (MaSp2) gene (GenBank accession no. EF595245) and mRNA (GenBank accession no. DQ409058); Av-UTR was determined by alignment of A. ventricosus minor ampullate spidroin gene (GenBank accession no. JX513956) and A. ventricosus major ampullate gland dragline silk protein-2 (F2) mRNA (GenBank accession no. AY177203).